CAD-SGE


This suite of tools enables the design of Synthetic Genetic Elements for broad host-range expression across diverse bacteria and yeast.

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Ribosome Binding Sites

Generate synthetic 5' UTRs/Ribosome Binding Sites with constraints adapted for versatile microbial expression.






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CDS Optimization

Our CDS optimization algorithm accounts for conserved structural elements, in particular at the N-terminus that facilitate high level expression across diverse microbial species. A custom 5' UTR/Ribosome Binding Site is generated concurrently.

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Operon Generation (iDOG)

Generate a synthetic multi-gene operon, appended with expression signals to enable expression across diverse prokaryotic microbes + yeast.